Sometimes it will show right after the first few cylinder rotations (either dry fire or live fire). Depending on how many times the cylinder has been opened and closed, it can show up as a partial turn line without any firing, just because the cylinder wasn't exactly indexed when swung shut so had to be rotated a bit (against the cylinder latch) to line up a cylinder notch.
Especially on a blued gun, it can appear very quickly with the first bit of use. And even on a stainless one, it will show pretty quickly with use.
IMO, it is not an useful indicator of usage at all. Even for a given model, it will vary a lot, since the cylinder latch beveling may be a little different from one gun to the next, the cylinder latch plunger spring tension may be a bit different from one gun to another - just not something that usefully tells you much about how much use a gun has seen. Especially after the first couple of hundred rounds, you could not use it to tell a gun with hundreds of rounds through it from one with thousands.
Depending on how many test rounds are fired by the manufacturer, a factory new gun may come with a faint drag line already in place.